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Image Search Results
Journal: bioRxiv
Article Title: Probing Ligand and Cation Binding Sites in G-Quadruplex Nucleic Acids by Mass Spectrometry and Electron Photodetachment Dissociation Sequencing
doi: 10.1101/563627
Figure Lengend Snippet: A) ESI-MS spectrum of 10 µM 23TAG in 0.2 mM KCl/100 mM TMAA. B) ESI-MS spectrum of 10 µM 23TAG + 10 µM PhenDC3 in 1.0 mM KCl/100 mM TMAA. C-D) ESI-MS spectra of the same solutions as A-B, with 0.75% (vol) sulfolane added. E-F) Bi-dimensional separation (mass spectrometry on x-axis and ion mobility drift time separation on y-axis) of spectra C-D. The arrival time following drift tube separation is proportional to CCS/z (CCS = collision cross section). Charge state distributions having the same CCS are therefore found on diagonals. Ions of the most compact conformations travel faster.
Article Snippet: Final solutions at the exact desired concentrations were prepared in nuclease-free grade water with
Techniques: Mass Spectrometry